angiogenic factors  (Cusabio)

Journal: Materials Today Bio

Article Title: Urine-derived stem cells efficiently assemble into micro-bone organoids supported by decellularized bone matrix microparticles for rapidly repairing bone defects through direct filling and paracrine functions

doi: 10.1016/j.mtbio.2025.102533

Figure Lengend Snippet: mRNA-seq reveals the in vitro formation mechanism of uBOs. (A) Schematic diagram of the research design. (B) Volcano plot depicting DEGs profiles in uBOs compared to USCs@DBM-MPs. (C) Heatmap analysis of the DEGs. (D) GO enrichment analysis of osteogenic and angiogenic related biological processes based on upregulated DEGs in uBOs. (E) KEGG analysis of osteogenic and angiogenic related signaling pathways using the upregulated DEGs in uBOs. (F) Heatmap analysis and GSEA analysis of ossification and blood vessel morphogenesis.

Article Snippet: Subsequently, the supernatants were harvested and used to quantify the levels of angiogenic factors ( ANG-5 , VEGF-C ) and osteogenic factors ( IGF-2 , OPG ) using ELISA kits (Elabscience or Cusabio, China), following the manufacturer's instructions.

Techniques: In Vitro, Protein-Protein interactions

Journal: Materials Today Bio

Article Title: Urine-derived stem cells efficiently assemble into micro-bone organoids supported by decellularized bone matrix microparticles for rapidly repairing bone defects through direct filling and paracrine functions

doi: 10.1016/j.mtbio.2025.102533

Figure Lengend Snippet: uBOs can stimulate osteogenesis and angiogenesis through paracrine mechanisms. (A) Schematic diagram of the research design. (B) ELISA was performed to quantify osteogenic cytokines ( OPG , IGF-2 ) and angiogenic cytokines ( VEGF , ANG-5 ) in uBOs-CM, USCs-CM, and CTL-CM. (C) ALP and ARS staining showed the stimulating effects of uBOs-CM, USCs-CM, and CTL-CM on the osteogenic differentiation of BMSCs. Scale bar = 250 μm. (D, E) The expression levels of osteogenic markers ( RUNX2 , OCN ) were assessed by qRT-PCR and WB in each group. (F) Transwell and tube formation assays showed the chemotactic and tube formation stimulating effects of uBOs-CM, USCs-CM, and CTL-CM on HUVECs. Scale bar = 200 μm. (G, H) The expression levels of angiogenic markers ( HIF-1α , VEGF ) were evaluated by qRT-PCR and WB in each group. Data are presented as mean ± SD (n = 3). p-values are calculated using one-way analysis of variance (ANOVA) with Bonferroni post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, and “ns” indicates no significance.

Article Snippet: Subsequently, the supernatants were harvested and used to quantify the levels of angiogenic factors ( ANG-5 , VEGF-C ) and osteogenic factors ( IGF-2 , OPG ) using ELISA kits (Elabscience or Cusabio, China), following the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Expressing, Quantitative RT-PCR

Journal: Materials Today Bio

Article Title: Urine-derived stem cells efficiently assemble into micro-bone organoids supported by decellularized bone matrix microparticles for rapidly repairing bone defects through direct filling and paracrine functions

doi: 10.1016/j.mtbio.2025.102533

Figure Lengend Snippet: Evaluation of the in-vivo biological functions of uBOs and its in-vivo tracing. (A, B) After 3 weeks of modeling, immunofluorescence staining was employed to assess the expression levels of osteogenic markers ( RUNX2 , OCN ) and angiogenic markers ( CD31 , VEGF ), and semi-quantitative analysis was performed. Scale bar = 1 mm or 250 μm. (C) qRT-PCR was used to quantitatively detect the gene expression levels of osteogenic markers ( RUNX2 , OCN ) and angiogenic markers ( PDGF-BB , VEGF ) in newly formed bone tissue. (D) After 6 weeks of implantation of uBOs, in-vivo cell tracking was performed using immunohistochemistry. In the uBOs group, more human nuclei-stained cells were detected within the bone trabeculae. Scale bar = 200 μm. Data are presented as mean ± SD (n = 6). p-values are calculated using one-way ANOVA with Bonferroni post hoc test. ∗p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, and “ns” indicates no significance.

Article Snippet: Subsequently, the supernatants were harvested and used to quantify the levels of angiogenic factors ( ANG-5 , VEGF-C ) and osteogenic factors ( IGF-2 , OPG ) using ELISA kits (Elabscience or Cusabio, China), following the manufacturer's instructions.

Techniques: In Vivo, Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Gene Expression, Cell Tracking Assay, Immunohistochemistry

Journal: Annals of Clinical and Translational Neurology

Article Title: Angiopoietin‐2 associates with poor prognosis in Moyamoya angiopathy

doi: 10.1002/acn3.52076

Figure Lengend Snippet: CSF concentrations of angiogenic (A) and inflammatory (B) protein factors released in CSF of MA patients and CTRL subjects with unrelated cerebrovascular diseases. Angiopoietin‐2 (Ang‐2), matrix metalloproteinase‐9 (MMP‐9) chemokine (C‐C motif) ligand 5 (CCL5/RANTES), Vascular Endothelial Growth Factor‐A (VEGF‐A), interleukin 8 (IL‐8/CXCL8), and interleukin 6 (IL‐6) were evaluated by ELISA and expressed as pg/mL or ng/mL, as indicated. The boxes represent data obtained in the range 25th–75th percentile; the line across the boxes indicates the median value; the lines above and below the boxes indicate extreme values (10th or 90th percentile). The statistical significance (* p < 0.05; ** p < 0.01; **** p < 0.0001) was calculated through nonparametric Mann–Whitney U test. Values of at least three independent experiments are shown. For each of the investigated proteins, numerically homogeneous groups of MA patients and CTRL subjects were compared. (C) The receiver‐operating characteristic (ROC) curve for circulating Ang‐2 on discriminating MA patients from CTRL subjects with unrelated cerebrovascular disease; AUC, area under the ROC curve.

Article Snippet: The Human Angiogenic Growth Factors & Angiogenesis Inhibitors RT 2 Profiler PCR Array (330231/PAXX‐024Y, Qiagen) was used.

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY